The molecular biology workbench for the modern lab

Gibson assembly, Golden Gate, CRISPR design, BLAST, protein analysis, codon optimisation, phylogenetic trees, virtual gels, and 70+ more tools — all in your browser.

Try in Browser (Beta) Download Desktop App Watch demos →
80+ Molecular tools
42 Codon-optimised organisms
8+ File formats supported
0 Account required for most tools

Capabilities

Everything the bench needs

From plasmid design to protein analysis, Biosmith covers the full molecular biology workflow in one place.

Cloning & Assembly

Gibson assembly, Golden Gate (BsaI/BbsI), MoClo Level 1 Plant, traditional ligation, and restriction enzyme cloning. Full primer generation, domestication check, and IDT CSV export for every workflow.

CRISPR & Design

Guide RNA design with off-target scoring for SpCas9, SaCas9, and Cpf1. CAR-T construct designer. VHH nanobody CDR annotator. Synthetic fragment designer with IDT gBlocks and Twist export.

Sequence Analysis

Interactive restriction digest map with single-cutter filter. 6-frame ORF finder. NCBI BLAST search with result import. Neighbour-joining phylogenetic tree. FASTQ per-base quality viewer. Repeat and motif finder.

Protein Tools

Kyte-Doolittle hydrophobicity plot. Chou-Fasman secondary structure prediction. Multiple sequence alignment with BLOSUM62 colour coding. Codon optimiser with CAI scoring for 42 organisms including all major expression systems.

Lab Utilities

SantaLucia 1998 Tm calculator. Virtual gel electrophoresis with 1kb+ ladder. Colony PCR planner. Sanger trace mismatch viewer. Buffer and dilution calculators. Provenance tracker for construction history.

File Formats

Import and export GenBank (.gb/.gbk), FASTA, EMBL, GFF3, SnapGene .dna, Plasmidsaurus .zip, and AB1 Sanger trace. Round-trip annotation fidelity. Drag-and-drop file loading.

All tools included

Gibson Assembly Golden Gate MoClo Level 1 Ligation Simulator Restriction Cloning CRISPR Guide RNA CAR-T Designer VHH Analyser SDM Designer Annotation Transfer Plasmidsaurus Import BLAST Search ORF Finder Phylogenetic Tree FASTQ Viewer Repeat Finder Motif Search Restriction Map Codon Optimiser Hydrophobicity Plot Secondary Structure MSA Alignment Protein Properties Tm Calculator Virtual Gel Colony PCR Sanger Trace Batch Primer Design Lab Calculator Synthetic Fragments RNA Structure ELN Notebook Construct Validator Reverse Translator Gene Lookup Gateway Cloning SeqDiff Gel Simulator Provenance Viewer

Open Beta

Join the beta

Biosmith is in active development. We're looking for molecular biologists to try it and give feedback.

Open Web App

Free during beta. No account required for most tools.

Desktop App

Desktop App

The desktop app unlocks AI annotation, Sanger trace analysis, IDT ordering, and lab archive features.

AI-assisted sequence annotation
Sanger trace analysis (.ab1)
IDT ordering integration
Local lab archive and search
Offline access to all 80+ tools
SnapGene .dna file compatibility

Desktop builds coming soon — join the beta waitlist above to be notified.

macOS · Windows · Linux — builds in preparation